Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Quality Biological Inc TE pH 8.0, 1000 mL
TE is composed of buffering agent Tris, and chelating agent EDTA. ¿ EDTA prevents degradation of DNA and RNA by chelating magnesium (or other divalent metal ions). ¿ Together, this Tris-EDTA based solution solubilizes DNA or RNA, while protecting it from degradation. ¿ Applications: TE is useful for reconstituting nucleic acids, during DNA precipitation and other DNA applications, storing and diluting DNA and RNA, breaking protein-links in immunohistochemistry procedures, and unmasking antigens and epitopes in formalin-fixed and paraffin-embedded tissue sections. ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase, RNase and Protease Tested ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Bioworld TBE Sample Buffer (6X), 50 mL
Product DescriptionTBE (Tris-Borate-EDTA) is a sample buffer used for nucleic acid analysis. It has a 6X concentration, 270 mM Tris base, 270 mM boric acid, 40% glycerol, 0.025% bromophenol blue G, 0.025% xylene cyanol, and 6 mM of EDTA.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
Kd Medical Inc TRIS BUFF SALINE 10X,PH7.4,1L
Tris Buffered Saline 10X is ready to use for dilution to a 1X working solution (pH 7.4). Tris Buffered Saline (TBS) is a pH stabilizing solution used for western blot and ELISA procedures. One 1 liter bottle.
National Diagnostics TRIS-GLYCINE ELECTROBLOTTING B
Tris-Glycine Electroblotting Buffer (10X), Stable, economical, ultra pure transfer buffer for Western Blotting procedures. 18 megOhm water. 0.2 micron filtration, 1 LT
eMolecules 1363382-91-5 | tert-butyl 3-(hydroxymethyl)-3-methylazetidine-1-carboxylate | MFCD20926177 | 1g
ChemScene | 5-Bromo-1H-pyrazole-4-carbonitrile | 100mg | 642098150 | CS-0196853 | 1703808-52-9 | MFCD28519385 | 171.985 | C4H2BrN3
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![eMolecules 1330764-06-1 | tert-butyl 7-(hydroxymethyl)-5-oxa-2-azaspiro[3.4]octane-2-carboxylate | MFCD20230641 | 1g](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502158274.JPG-250.jpg)
eMolecules 1330764-06-1 | tert-butyl 7-(hydroxymethyl)-5-oxa-2-azaspiro[3.4]octane-2-carboxylate | MFCD20230641 | 1g
Medchem Express | SG3199 | 1mg | 649421525 | HY-101161 | 1595275-71-0 | 584.673 | C33H36N4O6
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Alkali Scientific CellPro™ TE, 1X (Tris EDTA), pH 8.0, 100mL
Introducing CellPro™ TE 1X (Tris EDTA), meticulously formulated by Alkali Scientific™ to meet the exacting standards of molecular biology research. This Molecular Biology Grade TE buffer ensures exceptional purity and performance, making it an indispensable tool for various molecular biology applications, including DNA and RNA purification, DNA sequencing, and PCR. With its precisely balanced composition and stringent quality control measures, CellPro™ TE 1X provides researchers with reliable and reproducible results, facilitating accurate and efficient laboratory workflows. Trust CellPro™ TE 1X for your molecular biology experiments and experience the assurance of superior quality and performance in every assay.
Teknova TMN200 Solution, pH 8.0, Research Grade. 2L, Sterile 2 Pack.
200mM Sodium Chloride, 1mM Magnesium Chloride,20mM Tris
eMolecules 1179360-20-3 | TERT-BUTYL 3-(HYDROXYMETHYL)-1,4-DIAZEPANE-1-CARBOXYLATE HCL | MFCD23702017 | 1g
Ambeed | (R)-Oxiran-2-ylmethyl 4-nitrobenzenesulfonate | 1g | 628567785 | A635502 | 123750-60-7 | MFCD08460169 | 259.230 | C9H9NO6S
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Sigma Aldrich Fine Chemicals Biosciences mPAGER 4-12 Bis-Tris Preca
mPAGE 4-12% Bis-Tris Precast Gel 10x8 cmUnless otherwise stated in our catalog or other company documentation accompanying the product(s) our products are intended for research use only and are not to be used for any other purpose which includes but is not limited to unauthorized commercial uses in vitro diagnostic uses ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
eMolecules 1184985-22-5 | tert-butyl 3-(hydroxymethyl)-4-methyl-pyrrolidine-1-carboxylate | MFCD08461237 | 1g
ChemScene | 3-Hydroxy-5-(trifluoromethyl)benzonitrile | 100mg | 712788143 | CS-0373293 | 1243459-56-4 | MFCD16999150 | 187.121 | C8H4F3NO
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![Alkali Scientific TAE 10X Solution [TRIS Acetate EDTA 10X Solution], 10 Liters](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660482.jpg-250.jpg)
Alkali Scientific TAE 10X Solution [TRIS Acetate EDTA 10X Solution], 10 Liters
TAE 10X Solution is a ready-to-use buffer for electrophoresis applications, specifically designed for DNA analysis. The 10-liter solution is concentrated to 10X strength, making it highly efficient for routine gel electrophoresis, particularly for the separation of large DNA fragments. This buffer contains TRIS, acetate, and EDTA, which help maintain the stability of DNA during electrophoresis by preventing degradation. The 10X concentration allows users to easily dilute the buffer to a working strength, reducing preparation time and cost. TAE is widely used in laboratories for DNA analysis, PCR product analysis, and DNA sequencing applications, ensuring high resolution and reproducibility in gel electrophoresis.
Crescent Chemical Co Inc SERVA TRIS-GLYCINE/SDS BUFFER
SERVA Tris-Glycine/SDS electrophoresis buffer (10x) Running buffer for SDS PAGE. Supplied as 10 x concentrate. Contains 250 mM Tris, 1.92 M glycine, 1 % SDS. Size - 1L Storage Conditions - +15 °C TO +30 °C Catalog Number - 42529.01